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. Author manuscript; available in PMC: 2022 Mar 26.
Published in final edited form as: J Vis Exp. 2021 Mar 26;(169):10.3791/62045. doi: 10.3791/62045

Figure 13: Imaging tissue at 12 kV using various pixel dwell times and beam intensities.

Figure 13:

All images were collected using a 12 kV beam, beam intensity is on a device-specific scale ranging from 1 to 20. The field imaged is of the vascular lumen containing white and red blood cells. At 12 kV, imaging is optimized by adjusting pixel dwell time and beam intensity. Charging is reduced/absent at shorter pixel dwell times while cellular detail and image contrast are best with a longer pixel dwell time and higher beam intensity.