Figure 7: Limbal vasculature and associated cells in the peripheral mouse cornea.

A single image (A) from a 3D image block (B) can be seen through which a vessel, nerve bundle, and associated cells travel. Panel C shows a reconstructed vessel (red) with an associated pericyte (gray) wrapped around it covering the endothelial cell junctions. A nerve bundle (blue) bifurcates in close proximity to this vessel as it travels through the tissue. A neutrophil (yellow) can be seen parallel to the long axis of the vessel, with its polymorphic nucleus visible within its cell body and the trailing uropod visible as a protrusion towards the right of the image. A mast cell (magenta) is visible on the underside of the vessel. Panel D isolates this mast cell, where its granules (green) can be more easily seen overlaying the nucleus (purple) within the cell. Panel E highlights the cellular structures overlaid on the cellular reconstructions, with endothelial nuclei denoted in blue, and adherent microparticles visible in the vessel lumen (orange). Arrows show cell-cell borders between endothelial cells, which can be further seen as raised ridges extending along the cells on the luminal side of the vessel. Panel A scale bar = 2 μm. The image block used to reconstruct these cells is 30×30 μm in the x & y axis, and 42.5 μm in the z axis with a voxel resolution of 14.6×14.6×100 nm.