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. Author manuscript; available in PMC: 2022 Mar 26.
Published in final edited form as: J Vis Exp. 2021 Mar 26;(169):10.3791/62045. doi: 10.3791/62045

Figure 8: Reconstructed vascular network of the non-human primate retinal nerve fiber layer.

Figure 8:

(A) A 200×200 μm SBF-SEM image of the primate retina taken at 8192×8192 px. The location sampled is ~500 microns from the inferior temporal rim margin of a healthy eye with no pathology. The image series reconstructed in panels C & D were captured at 2048×2048 px, with imaging paused so that regions of interested could be imaged at 8192×8192 px. Panel B is the inlayed region of panel A, taken directly from the original image. Note the large number of axons and their mitochondria. (C) Orthoslice section through a 200×200×200 μm tissue volume of a control eye inferior temporal nerve fiber layer, with vasculature segmented. (D) Z-projection of the nerve fiber layer vasculature. This series illustrates the resolution possible in a large field using this methodology. Panel A scale bar = 20 μm. Panel B scale bar = 2 μm. Image series voxel resolution is 97.6×97.6×500 nm. Region of interest pixel resolution is 24.4×24.4 nm.