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. Author manuscript; available in PMC: 2022 Mar 26.
Published in final edited form as: J Vis Exp. 2021 Mar 26;(169):10.3791/62045. doi: 10.3791/62045

Figure 9: Segmentation and 3D volume rendering of vessels in the giant danio (Devario malabaricus) compact heart.

Figure 9:

(A) Two-dimensional micrograph in an image stack, showing the profile of a central venular-size vessel (arrow) and an endothelial nucleus (arrowhead), with surrounding cardiac myocytes rich in mitochondria and well organized sarcomeres (*). (B) Two-dimensional micrograph of the image stack with a capillary-size vessel (arrow). (C) Biorthogonal projections of the micrograph stack showing the capillary in panel B projected through one orthogonal slice. (D) 3D rendering of segmented endothelial cells lining the reconstructed vessel. Illustrated in green, red, blue, and purple are four separate endothelial cells; the endothelial cell depicted in blue can be seen in cross section in panel B (arrow), while the endothelial cells depicted in red (arrow) and green (arrowhead) are seen in cross section in panel A. Panels A & B scale bar = 2 μm. The image block reconstructed is 30×30 μm in the x & y axis, and 16 μm in the z axis with a voxel resolution of 14.6×14.6×100 nm.