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. Author manuscript; available in PMC: 2021 Jun 24.
Published in final edited form as: Nat Metab. 2020 Sep 17;2(9):974–988. doi: 10.1038/s42255-020-00273-8

Fig. 2 |. Lack of Fgr prevents At-CM polarization of BMDMs.

Fig. 2 |

a–c, BMDMs from WT and FgrKO mice were cultured o/n in the presence of AT-CM, LPS or LPS + IFN-γ; iNOS, Lysotracker and BODIPY were determined by flow cytometry. Representative histograms of iNOS, Lysotracker and BODIPY (a). Red, FgrKO; black, control. Mean fluorescence intensity (MFI) quantifications (b–d). e, Representative images of BMDMs treated as indicated o/n and subsequently stained with DAPI (blue) and BODIPY 493 (green). Scale bars: 20 μm. f, Lipid droplet quantification in BMDMs after polarization in the indicated conditions. a–d, Data are from n = 8 independent experiments; e and f, n = 5 experiments. For image analysis, more than 100 cells were analysed per experiment and averaged for quantification purposes. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Statistical analyses were performed using two-way ANOVA and Tukey’s post hoc test. Each point represents a biological replicate and data are shown as the mean ± s.e.m.