Fig. 1.

Expression of TRPP1 and PC1 in intact renal vessels and vascular smooth muscle cells (RVSMCs) of neonatal pigs. A and B: agarose gels demonstrating amplification of TRPP1 (bp: 314) and PC1 (bp: 352) in renal vessels and RVSMCs. Western immunoblotting with: C: a goat anti-TRPP1 antibody (EB09540; Everest Biotech, Ramona CA) and D: a mouse monoclonal anti-PC1 antibody (C1749; full length: FL; Sigma-Aldrich, St. Louis, MO) showing expression of full-length TRPP1 and PC1 proteins. Whereas TRPP1 bands ran slightly lower (~90 kDa) than its expected molecular weight (106 kDa), PC1 bands correspond to the approximate size (460 kDa). RVSMCs were individually selected using a patch pipette, as previously described (Soni et al., 2017b). NuPAGE Tris-Acetate (3–8%) gels and HiMark protein standard (Invitrogen, Carlsbad CA) were used to separate the large molecular weight full-length PC1 protein.