Skip to main content
. 2021 Apr 12;218(2):iyab060. doi: 10.1093/genetics/iyab060

Figure 3.

Figure 3

Characteristics of pol2rc-ΔΝ strains. (A) Small colonies after tetrad dissection (Figure 1B) give large and small colonies when re-streaked onto the YPRGAU medium. The photo on the left is a wild-type strain (the photo taken on day 3), and on the right—is the pol2rc-ΔN strain (photo taken on day 4). (B) Sensitivity to hydroxyurea (HU). Serial 10-fold dilutions of cell suspension were plated in horizontal rows by a 48-prong device on control or HU-containing YPDAU plates, and photos were taken on day 2. (C) DAPI-stained cells from small pol2rc-ΔN colonies that were shown in (B) in comparison to wild-type cells. The truncation of POL2 led to aberrant cells with irregular distribution of nuclear material, daughter cells without nuclei, and abnormally large or weird-shaped cells.