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. Author manuscript; available in PMC: 2022 May 24.
Published in final edited form as: J Chem Inf Model. 2021 Apr 26;61(5):2368–2382. doi: 10.1021/acs.jcim.1c00056

Figure 2.

Figure 2.

The effect of mhc_epitope weight on deimmunization of human erythropoietin (PDB ID 1EER). Designs (n=1000) were generated with fixed backbone Rosetta design with varying mhc_epitope weights, and the effect on six metrics evaluated: (A) REF2015 score, (B) unweighted mhc_epitope score, (C) PackStat score, and the number of (D) buried unsatisfied hydrogen bonds, (E) positively charged residues, and (F) negatively charged residues. Weights of 3.0, 5.0, and 10.0 were also tested, but were similar to a weight of 2.0 and are thus omitted for clarity. The green dashed line indicates the wild-type value (relaxed, but with no design). See also Figure S1 and Figure S2 for corresponding plots for other protein targets.