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Intradomain conformational dynamics during nucleotide binding and incorporation. (A–H) Changes in CPM fluorescence upon excitation at 290 nm following rapid mixing of a preincubated solution of an intradomain Dpo4 mutant (200 nM) and either DNAOH (300 nM,black traces) or DNAH (300 nM, red traces) with dTTP (1 mM) at 20 °C.
