. Author manuscript; available in PMC: 2021 Jun 24.

Published in final edited form as: Biochemistry. 2016 Oct 10;55(41):5832–5844. doi: 10.1021/acs.biochem.6b00878

Table 1.

Distances between Trp Donor Residues and Cys-Conjugated CPM Acceptor Fluorophores Projected from X-ray Crystal Structures

		distance^a (Å)
domain	mutant	apo^b	binary^c	ternary^d
Little Finger	Y274W-R267C^CPM	10.8	10.7	10.8
	Y274W-E291C^CPM	28.3	28.3	28.4
	Y274W-K329C^CPM	28.6	28.4	28.4
Thumb	Y224W-K172C^CPM	16.3	16.2	16.4
	Y224W-K212C^CPM	14.5	14.8	14.8
Palm	Y118W-K137C^CPM	26.6	26.1	25.8
Finger	S22W-K56C^CPM	22.0	20.9	21.1
	S22W-V62C^CPM	24.8	24.5	24.3

Estimated distances are calculated from crystal structures and do not account for the flexible linker utilized to attach the CPM fluorophore to the introduced Cys residues.

Distances calculated between the C_α atoms of residues in the apo structure of Dpo4 (PDB entry 2RDI).

Distances calculated between the C_α atoms of residues in the Dpo4·DNA binary complex structure (PDB entry 2RDJ).

Distances calculated between the C_α atoms of residues in the Dpo4·dideoxy-DNA·dATP ternary complex structure (PDB entry 2AGQ).