Table 2.

Antibacterial effects of O. elongatum.

Use part	Extracts	Used method	Tested strains	Key results	References
Leaves	Ethanolic extract	Method of diffusion in solid medium Macromethod of dilution in liquid medium	P	—	[1]
			P3	Ф = 10 ± 0.8 mm
			P65	Ф = 11 ± 0.8 mm
			P381	Ф = 8.5 ± 0.4 mm
			P2	Ф = 9 ± 0.8 mm
			P5	Ф = 8.33 ± 0.8 mm
Leaves	Aqueous extract		P	Ф = 11.66 ± 0.47 mm
			P3	Ф = 11 ± 0.95 mm
			P65	Ф = 11.83 ± 0.23 mm
			P381	Ф = 9.33 ± 1.24 mm
			P2	Ф = 10 ± 0 mm
			P5	Ф = 9.5 ± 0.4 mm
Aerial parts	Essential oil	Agar diffusion methods, broth microdilution assay	Staphylococcus aureus	Ф = 35.67 ± 0.66 mm	[3]
				MIC = 0.03%
				MMC = 0.13%
			Escherichia coli	Ф = 26.33 ± 1.66 mm
				MIC = 0.03%
				MMC = 0.03%
			Pseudomonas aeruginosa	Ф = 9.33 ± 0.66 mm
				MIC = 0.5%
				MMC = 0.5%
Leaves	Ethanolic extract	Agar-well diffusion assay	Escherichia coli	Ф = 30.33 ± 2.51 mm	[11]
Leaves	Essential oil	Disk-diffusion assay Microtitration method	Salmonella CECT 915	Ф = 34.33 ± 4.04 mm	[6]
				MIC = 0.0625%
				MBC = 0.0625%
			Salmonella S64	Ф = 28.17 ± 1.61 mm
				MIC = 0.0625%
				MBC = 0.125%
			E. coli O157 : H7 CECT4267	Ф = 19.67 ± 1.15 mm
				MIC = 0.25%
				MBC = 0.5%
			L. monocytogenes CECT4031	Ф = 34.00 ± 0.00 mm
				MIC = 0.125%
				MBC = 0.0625%
			L. monocytogenes L23	Ф = 31.00 ± 3.46 mm
				MIC = 0.5%
				MBC = 0.5%
Flowering tops	Essential oil	Disk-diffusion assay Microtitration method	Salmonella CECT 915	Ф = 31.50 ± 2.78 mm	[6]
				MIC = 0.0625
				MBC = 0.125%
			Salmonella S64	Ф = 28.17 ± 1.61 mm
				MIC = 0.0625%
				MBC = 0.125%
			E. coli O157 : H7 CECT4267	Ф = 18.00 ± 0.00 mm
				MIC = 0.25%
				MBC = 0.5%
			L. monocytogenes CECT4031	Ф = 29.00 ± 1.73 mm
				MIC = 0.125%
				MBC = 0.25%
			L. monocytogenes L23	Ф = 31.00 ± 3.46 mm
				MIC = 0.5%
				MBC = 0.5%
Flowering tops	Essential oil		Total mesophilic aerobic flora (FMAT)	Significant effect on microbial growth	[10]
Aerial parts	Essential oil	Agar-well diffusion method Microdilution assay	E. coli ATCC 25922	Ф = 21.33 ± 0.57 mm	[4]
				MIC = 0.5%
				MBC < 1%
			E. coli K12	Ф = 16.00 ± 1.00 mm
				MIC = 0.25%
				MBC = 0.5%
			B. subtilis DCM 6633	Ф = 24.66 ± 1.52 mm
				MIC = 0.5%
				MBC = 0.5%
			S. aureus ATCC 25923	Ф = 27.00 ± 1.73 mm
				MIC = 0.125%
				MBC = 0.125%
Aerial parts	Dissolved volatile fraction	Agar-well diffusion method Microdilution assay	E. coli ATCC 25922	Ф = 28.33 ± 0.57 mm	[4]
				MIC = 0.125%
				MBC = 0.25%
			E. coli K12	Ф = 17.00 ± 1.73 mm
				MIC = 0.125%
				MBC = 0.125%
			B. subtilis DCM 6633	Ф = 41.00 ± 2.64 mm
				MIC = 0.0625%
				MBC = 0.0625%
			S. aureus ATCC 25923	Ф = 30.00 ± 2.00 mm
				MIC = 0.0312%
				MBC = 0.0312%