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. Author manuscript; available in PMC: 2021 Jun 25.
Published in final edited form as: J Am Chem Soc. 2021 Jun 8;143(23):8902–8910. doi: 10.1021/jacs.1c03852

Figure 4.

Figure 4.

Design and in vitro click of dE2F. (A) A schematic diagram for the E2F motif and the sequence of N3-E2F-ODN. (B) Annealing of double strain N3-E2F-ODN. (C) N3-E2F-ODN was capable of binding with E2F1 transcription factor. (D) In vitro click of VHLL-BCN (#1 - #18, 150 μM) with N3-E2F-ODN (50 μM), which can be separated by 20% native PAGE. (E) Western blotting for E2F1 in HeLa cells after being treated with 10 μg/mL of dE2F (#1 - #18) for 12 h.