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. 2021 Jun 24;11:13208. doi: 10.1038/s41598-021-92416-4

Figure 2.

Figure 2

PLpro enzymology. The upper two panels show the initial rate of reaction for ubiquitin-rhodamine cleavage by PLpro in either tris-saline buffer or 0.8 M citrate. The lower two panels show the initial rate of substrate cleavage of a 7-mer peptide corresponding to the C-terminal of ubiquitin in either tris-saline buffer or 0.8 M citrate. The data points show the average of 4-replicates with error bars showing the standard deviation, with either a straight line or Michaelis–Menten fit shown as a solid black line.