Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jun 24;11:13251. doi: 10.1038/s41598-021-92784-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

CD86, MHCII, and CD206 identify M1- and M2-like cells in in vitro stimulated BMDM. (A) We applied gating strategies for M1/M2 that are commonly used in literature (i–iii) and an in-house approach (iv) to in vitro stimulated BMDM. (B) For in vitro polarization BMDM were differentiated for 5 days with M-CSF and then stimulated with LPS or IL-4 and IL-13. After 48 h cells were analyzed by flow cytometry and M1- (C) and M2-like cells (D) were quantified with each strategy from (A). (E) Duplicates were subjected to qPCR to determine expression of M1 (cd86, socs3, tnfa) and M2 (mrc1, cd200r1) genes. (n = 10 from 3 independent experiments). Kruskal–Wallis with post-hoc Dunn’s multiple comparisons test (C and D) or one way ANOVA with post-hoc Dunnett's (E) multiple comparisons test against medium *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.