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. 2021 Jun 24;11:13251. doi: 10.1038/s41598-021-92784-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Combination of phenotypical MNP subset analysis and functional distinction grants additional dimension to flow cytometric analysis. Flow cytometry of renal leukocytes (pre-gated on CD45 + Ly6G-, different cells are depicted schematically) is often either used to distinguish MNP subsets with specific surface markers (left box) or functionally distinct cells (right box). We propose a combined analysis of both dimensions for comprehensive understanding of functional and phenotypical state on a single cell level.