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. 2021 Jun 24;11:13251. doi: 10.1038/s41598-021-92784-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Different models of kidney injury possess a fingerprint-like MNP subset composition collectively but also in CD206 + and CD86 + MHCII + CD206- cells. (A) MNP subsets were quantified among total leukocytes (mid row) in different models of kidney injury (means of n = 9 for 7 days UUO sham control, n = 6 for IRI, n = 9 for UUO, n = 11 for Alport; cell number is given in cells per mg kidney). Our approach additionally allowed the quantification of functionally distinct CD206 + (top row) and CD86 + MHCII + CD206- (bottom row) cells in each MNP subset. (B) Quantification of cell numbers from (A) as bar graph. (C) Percent CD86 + and MHCII + cells among CD206 + cells. One-way ANOVA followed by Tukey’s multiple comparisons test of y = ln(y) transformed data *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.