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. 2021 Jun 24;12:3938. doi: 10.1038/s41467-021-23756-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a FRET signal between Bodipy-FL PIP2 (2.5 molar%) and Lumi-4 Tb attached to C255^6.27 of the wild-type receptor or of the GHSR-A204E mutant. The signal obtained with the S1,2,3 mutant is given for comparison. The signal was normalized to that measured with wild-type GHSR. b Changes in MB emission λ_max for wild-type GHSR, the A204E, and the S1,2,3 mutant in the absence or in the presence of 10 μM ghrelin. Data are mean ± SD of three experiments. Statistical values were obtained by means of unpaired Student’s t test (**0.001 < p < 0.01, ****p < 0.0001). Source data are provided as a Source data file.