Figure 2.

Requirement for YTHDF2 in NK cell antitumor immunity.(A–F)Ythdf2^WT and Ythdf2^ΔNK mice were injected with B16F10 cells (10⁵ i.v.). 14 d after injection, the mice were euthanized for postmortem analysis. Quantification of total metastatic nodules in the lungs from Ythdf2^WT (n = 7) and Ythdf2^ΔNK (n = 8) mice; gross morphology of individual lung lobes is shown in A. The percentage and absolute number of infiltrating NK cells in lung tissues from Ythdf2^WT and Ythdf2^ΔNK mice (n = 5 mice per group, at least two independent experiments [B and C]). IFN-γ, granzyme B, and perforin expression in the lung-infiltrated NK cells from Ythdf2^WT and Ythdf2^ΔNK mice (n = 5 mice per group, at least two independent experiments [D–F]). (G–L) 10⁶ IL-2–expanded NK cells from Ythdf2^WT and Ythdf2^ΔNK mice were injected i.v. into Rag2^−/−Il2rg^−/− mice. 1 d later, B16F10 cells (10⁵ i.v.) were injected into mice. 14 d after injection, mice were euthanized for postmortem analysis. Quantification of total metastatic nodules in the lungs from Ythdf2^WT and Ythdf2^ΔNK mice (n = 5 mice per group, at least two independent experiments [G]). The percentage and absolute number of infiltrating NK cells in the lung tissues (n = 5 mice per group, at least two independent experiments [H and I]). IFN-γ, granzyme B, and perforin expression in the lung-infiltrated NK cells from Ythdf2^WT and Ythdf2^ΔNK mice (n = 5 mice per group, at least two independent experiments [J–L]). Each symbol represents an individual mouse. Data are shown as mean ± SD and were analyzed by unpaired two-tailed t test (*, P < 0.05; **, P < 0.01; ***, P < 0.001). L, left; R, right.