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. 2021 Jun 24;19:96. doi: 10.1186/s43141-021-00195-2

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© The Author(s) 2021

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Fig. 2 — Different stages of maize cultures from inoculated nascent explants (immature embryo) (A) to fully developed hardened plants transferred into pots grown in poly house (J), after going through different stages of Agrobacterium-mediated transformation viz., co-cultivation (B), selection I (D), selection II (F), regeneration of plantlets (H), and rooting of plantlets (I); cultures without co-cultivation acts as controls at every stage (C, E, & G)