Table 2.

Survival frequency of in vitro cultures at three different stages and their frequency of regeneration in maize affected by different parameters with different variables involved in Agrobacterium-mediated transformation after co-cultivation

Parameter	Variable	Subculture	Selection-I	Selection-II	Regeneration
Optical density of bacterial culture	0.6	60.17 ± 5.13	28.59 ± 4.04	17.19 ± 3.51	8.94 ± 1.00
	0.8	86.93 ± 4.50	57.41 ± 7.76	37.09 ± 4.72	23.38 ± 3.51
	1	73.55 ± 9.50	46.00 ± 3.60	30.66 ± 5.29	19.55 ± 6.50
Strength of the infection medium	Half MS (plain)	87.93 ± 2.00	49.48 ± 5.50	40.51 ± 3.51	21.89 ± 3.51
	Full MS (plain)	83.20 ± 5.56	36.98 ± 8.08	28.11 ± 6.80	16.60 ± 3.78
	LS inf	74.92 ± 6.65	23.07 ± 5.00	14.46 ± 6.11	12.30 ± 5.03
pH of the infection medium	pH 5.2	50.00 ± 8.88	30.00 ± 2.64	18.88 ± 2.64	13.70 ± 2.08
	pH 5.4	70.30 ± 6.65	40.61 ± 2.64	29.69 ± 5.03	18.61 ± 2.08
	pH 5.8	82.57 ± 5.77	60.60 ± 8.50	39.84 ± 3.78	21.51 ± 5.03
Acetosyrengone concentration	0 μM	50.28 ± 4.16	33.71 ± 1.15	17.71 ± 3.05	9.71 ± 1.15
	100 μM	84.21 ± 5.68	63.28 ± 7.00	38.59 ± 2.51	23.59 ± 2.08
	200 μM	73.40 ± 2.51	56.13 ± 6.42	28.40 ± 5.50	18.86 ± 4.50
Macerozyme treatment	0.1%	57.16 ± 5.13	36.16 ± 3.78	21.33 ± 5.03	12.50 ± 3.00
	0.2%	42.83 ± 4.04	20.50 ± 6.55	17.00 ± 3.46	10.66 ± 2.51
	0.5%	28.83 ± 7.50	12.33 ± 6.42	10.16 ± 5.13	6.33 ± 5.03
Vacuum infiltration	0 min	83.33 ± 7.63	46.16 ± 4.17	34.16 ± 3.51	17.00 ± 2.00
	10 min	61.42 ± 9.07	33.28 ± 3.78	22.71 ± 4.58	11.28 ± 1.52
	15 min	58.62 ± 5.77	28.79 ± 2.08	16.72 ± 2.51	7.93 ± 1.15
Infection time	10 min	80.32 ± 7.63	54.91 ± 7.63	38.68 ± 5.50	22.78 ± 5.13
	15 min	70.66 ± 5.29	44.22 ± 3.21	22.44 ± 5.13	17.11 ± 0.57
	30 min	58.97 ± 5.00	33.33 ± 3.00	12.99 ± 5.03	6.32 ± 0.57
Co-cultivation period	2 days	84.00 ± 3.60	52.00 ± 9.16	36.66 ± 1.15	27.66 ± 1.52
	3 days	66.33 ± 9.01	36.66 ± 8.50	23.00 ± 3.46	15.83 ± 2.51
	4 days	30.65 ± 5.00	13.69 ± 1.73	15.00 ± 2.00	7.17 ± 1.00