Table 3.

Transformation frequency obtained on hygromycin regime and molecular screening of putative transgenics through PCR using hpt II primers of maize obtained through Agrobacterium-mediated transformation

No. of immature embryos co-cultivated	Survival frequency at subculture stage	Survival frequency in Selection-I	Survival frequency in Selection-II	Regeneration frequency	PCR +ves for hpt II	Transformation frequency (%)
5697	86.29 (4916)	71.19 (4056)	46.58 (2654)	29.1 (1658)	329	5.77

Numbers in parenthesis indicate total number of calli tested