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. 2021 Jun 24;11:13230. doi: 10.1038/s41598-021-92718-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Alkaline pH sensitivity of B. thailandensis ∆dbcA. (a) Growth rate of B. thailandensis strains (E264; vec, E264; DbcA, ∆dbcA; vec, and ∆dbcA; DbcA) at different times in Mueller Hinton broth (MH1) with 100 µg/ml Tmp. The error bars indicate standard deviations of three biological replicates. (b) Spot assay of E264; vec, E264; DbcA, ∆dbcA; vec, and ∆dbcA; DbcA on MH1 agar media. 1:10 dilutions of indicated strains were spotted on NaOH adjusted MH1 media with 100 µg/ml Tmp and 0.001% rhamnose. Plates were analyzed after 24 or 48 h at 37 °C. (c) Comparison of alkaline pH sensitivity of B. thailandensis ∆dbcA in MH2 with MH1 agar media. MH2 contains ~ 20 to 25 mg/l of calcium and ~ 10 to 12.5 mg/l of magnesium, whereas MH1 contains no added calcium and magnesium salts. The pH was adjusted by either HCl or NaOH. Plates were analyzed after 48 h of growth at 37 °C.