Table 1.
Bacterial strains and plasmids used in this study.
| Strains | Description | Source or reference |
|---|---|---|
| Escherichia coli W3110 | Wild Type, F−, λ−, IN (rrnD-rrnE)1, rph-1 | E. coli genetic stock center, Yale University |
| Escherichia coli W3110; vec | W3110 transformed with pBBR1MCS-2 | 15 |
| Escherichia coli ∆nhaA | W3110, ∆nhaA-737::kan | 22 |
| Escherichia coli BW25113 | Wild Type, lacIq rrnBT14 ∆lacZWJ16 hsdR514 ∆araBADAH33 ∆rhaBADLD78 | 91 |
| Escherichia coli BW25113 ∆cydB | Kanamycin-resistant Keio collection mutant | 92 |
| Escherichia coli BC202KS | W3110 ∆yqjA::TetR ∆yghB781::KanS | 15 |
| Escherichia coli BC202; vec | BC202KS transformed with pBBR1MCS-2 | This study |
| Escherichia coli ∆yqjA | W3110; ∆yqjA::TetR | 50 |
| Escherichia coli ∆yqjA; vec | ∆yqjA transformed with pBBR1MCS-2 | This study |
| Escherichia coli ∆yqjA; YqjA | ∆yqjA transformed with pRP102 | This study |
| Escherichia coli ∆yqjA; DbcA | ∆yqjA transformed with pRP101 | This study |
| Escherichia coli RHO3 | SM10(lambda pir), kanS; ∆asd::FRT ∆aphA::FRT | 93 |
| Burkholderia thailandensis E264; vec | E264 transformed with pSCrhaB2 TmpR | 15 |
| Burkholderia thailandensis E264 ΔdbcA; vec | ΔdbcA::FRT transformed with pSCrhaB2 TmpR | 15 |
| Burkholderia thailandensis E264; DbcA | E264 transformed with pSCdbcA | 15 |
| Burkholderia thailandensis E264 ΔdbcA; DbcA | ΔdbcA::FRT transformed with pSCdbcA | 15 |
| Escherichia coli WD102 | W3110, pmrAC, zjd-2211: Tn10 | 94 |
| Klebsiella pneumoniae ST258 | A clinical isolate from Public Health England resistant to polymyxin | 19 |
| Klebsiella pneumoniae arnT::Tn | A transposon insertion mutant carrying a chloramphenicol resistance determinant (T30) | 72 |
| Salmonella enterica subsp. Enterica 14028 | Virulent wild type strain | ATCC |
| Pseudomonas aeruginosa PAO1 | Wild type strain | 95, 96 |
| Vibrio cholerae C6706 | O1 El Tor strain | 97 |
| Serratia marcescens 13880 | Wild type strain | ATCC |
| Staphylococcus aureus subsp. aureus Rosenbach, 6538 | Wild type strain | ATCC |
| Bacillus subtilis 6051 | Wild type strain | ATCC |
| Burkholderia cenocepacia K56-2 | ET12 clone related to J2315, CF clinical isolate | BCRRC, B. cepacia Research and Referral Repository for Canadian CF Clinics |
| Burkholderia cenocepacia MH55 | K56-2, ΔarnT-arnBC+ lptGD31H (lptGS) | 98 |
| Plasmids | ||
| pBBR1MCS-2 | Expression vector; RK2 ori, lacZa, T3 and T7 promoters, KanR | 99 |
| pRP101 | pBBR1MCS-2 expressing dbcA | This study |
| pRP102 | pBBR1MCS-2 expressing EcyqjA | This study |
| pSCrhaB2 | Expression vector; oripBBR1rhaR, rhaS, PrhaBTmpRmob + | 100 |
| pSCdbcA | pSCrhaB2 expressing dbcA with His6 tag at C terminus | 15 |