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. 2021 Jun 24;11:13260. doi: 10.1038/s41598-021-92751-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Gestational administration of IL-6 to dams results in upregulation of JAK/STAT pathway in newborn kidneys. Western blot analysis showing changes in phosphorylation of JAK2, STAT3 and STAT5 in kidneys of pups born to dams treated with saline (Control) versus IL-6. Kidneys all newborns from each dam (n = 2, kidneys from 6 to 8 pups included in each sample) were pooled to obtain protein lysate for the Western blot. Increased phosphorylation of JAK2 and STAT3 but not STAT5 was observed. β-Actin was used as the loading control.