Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jun 24;12:3946. doi: 10.1038/s41467-021-24170-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a Genomic alterations of potentially targetable genes in AFPGC and TCGA-GC. b Overall frequency of selected targetable genes in AFPGC and TCGA-GC. c Representative images of ERBB2 and CCNE1 status. Presented data are a representative image of three independent experiments. Upper panel shows IHC score of ERBB2 with 0, 1+, 2+, and 3+. Lower panel shows representative FISH image of ERBB2 and CCNE1. Scale bar represents 50 μm. d The positive rate of ERBB2 and CCNE1 in AFPGC and TCGA-GC. e, f Associations between ERBB2 status and OS in AFPGC (e) or in TCGA-CIN (f). g, h Associations between CCNE1 status and OS in AFPGC (g) or in TCGA-CIN (h). i, j Associations between combined status of ERBB2 and CCNE1 and OS in AFPGC (i) or in TCGA-CIN (j). k, l Forest plot of multivariable Cox proportional hazard regression in AFPGC (k) or in TCGA-CIN (l). The hazard ratios are presented and the horizontal lines indicate the 95% confidence intervals. In AFPGC-WES and TCGA-GC, the positive status of ERBB2/CCNE1 was defined as amplification by GISTIC 2.0. In AFPGC-total, the definition of the positive status of ERBB2 and CCNE1 was shown in Supplementary Methods. Statistical significance was determined using two-sided χ²-test (d), log-rank (Mantel–Cox) test (e–j), and multivariate Cox regression (k, l). AFPGC-WES, the AFPGC cohort with WES data; AFPGC-total, the whole AFPGC cohort in this study; CCNE1^N, CCNE1 negative; CCNE1^P, CCNE1 positive; CI, confidence interval; ERBB2^N, ERBB2 negative; ERBB2^P, ERBB2 positive; FISH, fluorescence in situ hybridization; HR, hazard ratio; IHC, immunohistochemistry; neg, negative; OS, overall survival; pos, positive. Source data are provided as a Source Data file.