Fig. 1. Generating stem cell derived disease models and complex neuronal cultures based on patient iPSCs requires careful and extensive validation.

a Process flow to generate disease-specific stem cell lines from patients to model in vitro complex neurodevelopmental and neurodegenerative diseases using 3D spheroid cultures of cortical and motor neurons. b Stem cell-derived 3D spheroid neural cultures were dissociated and used to generate high-content data for culture and disease modeling. Cells are subsequently analyzed to generate specific culture breakdowns using PRISM for in-depth characterization of neural cultures via multiplexed staining.