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. 2021 Jun 24;4:786. doi: 10.1038/s42003-021-02276-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Representative images from the same area of a 1016A-derived motor neuron culture, stained with the optimized 12-marker PRISM antibody panel after being grown for 21 days as a 3D spheroid, then dissociated into 2D and maintained for 14 days. Scale bar is 100 microns.