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. 2021 Jun 24;12:3937. doi: 10.1038/s41467-021-24224-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a DNA fiber analysis of the replication fork speed and symmetry in nontreated conditions. Left: schematic of the CldU/IdU pulse-labeling protocol used. Middle: analysis of the replication fork speed. Total number of molecules analyzed: siCTRL (n = 362), siGANP (n = 407). The plot includes the mean ± SD. Statistical test: unpaired t-test with Welch’s correction, two-tailed. Right: analysis of the replication fork symmetry. The ratio between CldU/IdU was calculated from the same molecules used for the analysis of the replication fork speed (middle) (siCTRL n = 362, siGANP n = 407). Bounds of box are 25th–75th percentile, center shows the median, whiskers indicate the 10th–90th percentiles, data outside the range are drawn as individual dots. Statistical analysis: unpaired t-test with Welch’s correction, two-tailed. b DNA fiber analysis of the replication fork recovery upon HU removal. Left: schematic of the CldU/IdU pulse-labeling protocol used. Middle: length of the IdU tracks measured upon hydroxyurea removal. Total number of molecules analyzed: siCTRL (n = 190), siGANP (n = 181). The plot includes the mean ± SD. Statistical test: unpaired t-test with Welch’s correction, two-tailed. Right: analysis of the replication fork symmetry. The ratio between CldU/IdU was calculated from the same molecules used for the analysis of the length of the IdU tracks measured upon hydroxyurea removal (middle) (siCTRL n = 190, siGANP n = 181). Bounds of box are 25th–75th percentile, center shows the median, whiskers indicate the 10th–90th percentiles, data outside the range are drawn as individual dots. Statistical analysis: unpaired t-test with Welch’s correction, two-tailed.