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. 2021 Jun 25;6:240. doi: 10.1038/s41392-021-00562-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — The high expression of AFAP1-AS1 was positively associated with lung adenocarcinoma patients’ poor prognosis. a AFAP1-AS1 was highly expressed in 187 lung cancer tissues (89 lung adenocarcinoma and 98 lung squamous carcinoma), comparing with 36 paraffin-embedded normal lung tissues as determined by in situ hybridization (left, representative pictures of in situ hybridization, Scale bar: 20 μm or 50 μm; right, the statistical results according to in situ hybridization score values; right, red: high expression, green: low expression). b The correlation between AFAP1-AS1 expression and prognosis of patients with lung cancer (p < 0.0001, HR = 4.423) by Kaplan-Meier survival analysis. The prognosis of lung cancer patients with the high AFAP1-AS1 expression is significantly lower than that of lung cancer patients with the low AFAP1-AS1 expression. c High AFAP1-AS1 expression is positively correlated with distant metastasis in 86 patients with lung adenocarcinoma (left), but not in 88 patients with lung squamous carcinoma (right). Data are presented as the means ± SD