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. 2021 Jun 25;6:240. doi: 10.1038/s41392-021-00562-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — AFAP1-AS1 upregulates c-Myc protein level and inhibits c-Myc ubiquitination through binding to SNIP1. a The c-Myc expression was detected in A549 and PC9 cells after knockdown or overexpression of AFAP1-AS1 by western blotting. b Knockdown of SNIP1 attenuated the regulation of AFAP1-AS1-mediated upregulation of c-Myc protein. c No binding between AFAP1-AS1 and c-Myc showed by RNA pull down assay. d c-Myc expression was assessed in A549 and PC9 cells treated with 50 μg/ml CHX or 20 μM MG132 for 0–40 min after AFAP1-AS1 knockdown. The results showed that only MG132 abolished the AFAP1-AS1 knocked down-mediated downregulation of c-Myc. e The c-Myc ubiquitination was dramatically decreased in A549 cells after overexpression of AFAP1-AS1 using IP experiment. f The ubiquitination of c-Myc protein was abolished in A549 cells after co-transfection with His-SNIP1, the Flag-c-Myc vector and the pool of two siRNAs targeting AFAP1-AS1 using IP experiment