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. 2021 Jun 25;6:240. doi: 10.1038/s41392-021-00562-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — AFAP1-AS1 upregulates ZEB1, ZEB2, and SNAIL transcription through promoting the binding between SNIP1 to c-Myc. a Chromatin immunoprecipitation experiments showed that c-Myc protein binds to the promoter regions of ZEB1, ZEB2, and SNAIL genes and promotes their transcription in A549 cells. b qRT-PCR results showed c-Myc promoted ZEB1, ZEB2, and SNAIL transcription in A549 and PC9 cell lines, whereas knockdown of AFAP1-AS1 weakened the effect of c-Myc on the transcription of ZEB1, ZEB2, and SNAIL. c AFAP1-AS1 promoted the transcription of ZEB1, ZEB2, and SNAIL in A549 and PC9 cell lines, whereas knockdown of SNIP1 weakened the effect of c-Myc on the transcription levels of ZEB1, ZEB2, and SNAIL. d Overexpression of c-Myc induced ZEB1, ZEB2, and SNAIL expression at the protein level in A549 and PC9 cell lines, whereas knockdown of AFAP1-AS1 weakened the function of c-Myc on ZEB1, ZEB2, and SNAIL proteins by western blotting, which is consistent with their transcription levels. e AFAP1-AS1 enhanced the expression of ZEB1, ZEB2, and SNAIL proteins in A549 and PC9 cell lines, whereas knockdown of SNIP1 weakened the effect of c-Myc on ZEB1, ZEB2, and SNAIL proteins expression. All experiments were repeated at least three times. Data are presented as the means ± SD