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. 2021 Jun 24;4:782. doi: 10.1038/s42003-021-02323-7

Fig. 6. Effect of P4HA3 depletion on cell migration and growth in vitro.

Fig. 6

a Association between P4HA3 and prognosis in patients with non-small cell lung cancer. b Box-and-whisker plot of P4HA3 mRNA levels in non-tumor (n = 107), primary tumor (n = 1008), and recurrent tumor tissues (n = 2) from the lung cancer dataset in the Cancer Genome Atlas. **P < 0.01. c Effects of P4HA3 siRNA on the migratory activity in TGF-β-treated HCC827 cells. HCC827 cells were cultured in the presence of 2 ng/mL TGF-β for 2–5 weeks to induce EMT and transfected with P4HA3 siRNA for 48 h prior to the cell migration assay. Cells were observed by bright field microscopy at ×100 magnification. Scale bar, 500 μm. Data are presented as mean ± SD. **P < 0.01. d Effect of P4HA3 knockdown on cell viability in A549 cells. Cells were enumerated 72 h after transfection with P4HA3 siRNA. Data are presented as mean ± SD. **P < 0.01.