Table 2.

A Inter-day intermediate precision of PMF-EIC method with mAb X containing ~ 0.1% of E262K substitution. The % substitution is calculated using Eq. (1). B Accuracy of PMF-EIC method at ~ 400 pmol and ~ 8 pmol column load.

Intermediate inter-day precision	% E262K
A
Day 1	0.08
Day 2	0.12
Day 3	0.14
Day 4	0.10
Day 5	0.12
Day 6	0.09
Average	0.11
% CV	18.8

Column load	AUC of EK peptide	AUC of native peptide	%E262K	Average %E262K	% CV
B
~ 400 pmol	130,195,529	331,971,597	28.2	28.8	5.7
	128,506,560	296,820,826	30.2
	131,613,201	339,193,814	28.0
~ 8 pmol	3,463,371	2,633,558	56.8	47.5	44.3
	1,958,042	3,970,226	33.0
	1,918,275	1,717,359	52.8