Table 4.

Accuracy and precision of EK and native peptide obtained in triple-Q MS using SRM.

Peptide	LLOQ (0.065 pmol)		LQC (0.26 pmol)		MQC (1.302 pmol)		HQC (52.08 pmol)
	Estimated (pmoles)	Accuracy (%)	Estimated (pmoles)	Accuracy (%)	Estimated (pmoles)	Accuracy (%)	Estimate (pmoles)	Accuracy (%)
EK	0.082	26.71	0.273	5.13	1.325	1.80	46.796	− 10.15
	0.077	18.38	0.286	9.85	1.217	− 6.52	51.969	− 0.21
	0.063	− 2.57	0.287	10.22	1.320	1.41	53.371	2.48
	0.065	0.57	0.247	− 5.02	1.304	0.17	46.432	− 10.84
	0.080	23.07	0.264	1.54	1.334	2.45	48.453	− 6.96
	0.069	5.74	0.254	− 2.19	1.340	2.91	49.834	− 4.31
%CV	11.1		6.1		3.5		5.6

Peptide	LLOQ (126.2 pmol)		LQC (378.6 pmol)		MQC (504.7 pmol)		HQC (908.5 pmol)
Native	104.8	− 16.93	338.578	− 10.56	450.023	− 10.84	778.725	− 14.29
	137.3	8.84	345.075	− 8.85	475.883	− 5.72	739.915	− 18.56
	115.3	− 8.65	331.741	− 12.37	460.245	− 8.82	837.976	− 7.77
	111.0	− 12.00	339.177	− 10.40	460.111	− 8.84	828.069	− 8.86
	114.2	− 9.51	360.945	− 4.65	495.992	− 1.73	843.571	− 7.15
	108.9	− 13.69	338.206	− 10.66	515.043	2.04	844.758	− 7.02
%CV	9.9		2.9		5.2		5.3