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. 2021 Mar 15;137(25):3518–3532. doi: 10.1182/blood.2020008551

Figure 3.

Figure 3.

Knockdown of VLCAD alters mitochondrial metabolism and reduces leukemia cell engraftment. Quantification of PDH activity (A), ATP levels (B), and (C) mitochondrial mass of TEX wild-type and VLCAD-knockdown cells. (D) Schematic diagram of the study, highlighting injection of an equal number of TEX wild-type or D6 cells into the tail vein of NSG mice. Engraftment levels were quantified at an intermediate time point of 5 weeks, via BM, and at the planned end point at day 101. Created using BioRender. (E) Engraftment levels of TEX wild-type and VLCAD-knockdown cells at the end point. (F) Overall survival of mice engrafted with TEX wild-type or D6 cells. *P = .0134, log-rank (Mantel-Cox) test. *P ≤ .05, **P ≤ .002, ***P ≤ .001, 1-way ANOVA with Tukey’s post hoc test (A-C); Mann-Whitney U test (E). With the exception of (D), all data are mean ± standard deviation.