Fig. 7.
Structural property of apoE is affected by interaction with bacterial products. A: Increasing concentrations of LPS from Escherichia coli (LPSEc), LPS from Pseudomonas aeruginosa (LPSPa), or Lipid A from E. coli (LipA) were added to 5 μM apoE, and then, intrinsic fluorescence spectra were recorded. Figures show representative spectra from three independent experiments (n = 3). B: The binding curves were obtained reporting the shift in the λmax of apoE alone compared with apoE in the presence of increasing concentrations of bacterial ligands (0–100 μg/ml). C: Dissociation constants (Kd) of protein-ligand interaction were calculated from the binding curves. ∗P < 0.05. λmax, maximum absorbance; LPS, lipopolysaccharide; ns, not significant.