Figure 2.

Characterization of five neutralizing mAbs

(A) Neutralization of SARS-CoV-2 pseudovirus by mAbs 6-74, 3-5, 8-92, 10-121, and 9-105 in 293FT/ACE2/TMPRSS2, Calu-3 (human lung cancer cell line), and Caco-2 (human colon adenocarcinoma cell line) cells is shown. A non-neutralizing mAb, 8-38, was used as a negative control (orange open circle).

(B) Cell fusion of 293FT/DSP8-11/SARS-CoV-2-S cells with 293FT/DSP1-7/ACE2/TMPRSS2 and Calu-3/DSP1-7 cells was measured by luciferase activity at 6 and 20 h after coculture, respectively. A non-neutralizing mAb, 5-76, was used as a negative control (orange open circle).

(C) IC₅₀ values of the five mAbs are summarized.

(D) The binding and genetic characteristics of mAbs are summarized. Binding to RBD and NTD was analyzed by AlphaScreen. K_D values were determined by SPR analysis (Figure S1D). Gene usage (gene), somatic hypermutation % (SHM) of heavy and light chains, and CDRH3 amino acids (aa) and length were analyzed by IMGT vquest.

Data shown in (A) and (B) are represented as means ± SD (n = 3). See also Figure S1.