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. 2021 Jun 11;12:701009. doi: 10.3389/fphar.2021.701009

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Copyright © 2021 Zhang, Huang, Yao, Liu, Ruan, Nong, Chen, Fan, Wei, Wang, Sun, Li, Zhang, Zhang and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of gestational FA on pregnancy outcomes and offspring characteristics. FA administration during pregnancy had no significant effect on maternal body weight (A), length of gestation (B), dam litter size (C), fraction of pup sex (D), and offspring body weight during development (E). Two-way repeated measures ANOVA was used to analyze the maternal body weight: F (2, 18) = 0.2421, p = 0.7875. Length of gestation via One-way ANOVA: F (2, 27) = 0.2691, p = 0.7661. Dam litter size via One-way ANOVA: F (2, 27) = 0.1357, p = 0.8737. Fraction of pup sex via One-way ANOVA: F (2, 27) = 0.04937, p = 0.9519. Two-way repeated measures ANOVA was used to analyze the offspring body weight: F (2, 18) = 0.3204, p = 0.7299. Data are presented as scatter points plus the mean ± SEM. n = 10 for each group.