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. 2021 Jun 14;8:668340. doi: 10.3389/fmolb.2021.668340

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Copyright © 2021 Parra.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative graphs showing the J-function [J(r)] for two different cell populations, illustrating the cellular distribution between the two groups of cells using a representative point pattern in multiplex immunofluorescence images of lung adenocarcinoma. (A) Random point pattern distribution of cell phenotype A and B. (C) Graphic representation of the J(r) curve showing its lineup with the theoretical estimate (Poisson line), characterizing a random or mixed pattern of one cell population in relation to another cell population. (B) Unmixed point pattern distribution of cell phenotype A and B. (D) Graphic representation of the J(r) curve showing highly increased distance above the theoretical estimate (Poisson line), characterizing a clustering or unmixed pattern of one cell population in relation to another cell population. The graphics were generated using R studio software version 3.6.1.