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. 2021 Jun 14;8:668340. doi: 10.3389/fmolb.2021.668340

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Copyright © 2021 Parra.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative schema of hierarchical and divisive clustering to agglomerate similar objects into groups (A). Uniform manifold approximation and projection (UMAP) showing different cell population distribution patterns extracted from two different lung adenocarcinoma cases analyzed with multiplex immunofluorescence against malignant cells [cytokeratin (CK)+] and CD3+, CD4+, CD8+, PD-1+, PD-L1+, and CD68+ antibodies (B, C). The graphics B and C were generated using R studio software version 3.6.1.