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. 2021 Jun 11;11:691798. doi: 10.3389/fonc.2021.691798

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Copyright © 2021 Sun, Du, Kohli, Huang, Chen, Xu, Shen, Wang and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identity analysis of fraction 3 (large microvesicles) and fraction 5 (exosomes). (A) Transmission electron microscopy. The round shape of large microvesicles (LMV) and exosomes by negatively staining the background with phosphotungstic acid. The bar represents 200 nm. (B) Nanosight analysis. Particle sizes of fractions 3 and 5 are different with 405.5 nm and 100.3 nm in the main peak value, respectively. (C, D) Flow cytometry of characteristic protein analysis. Results of CD63 (C) and CD81 (D) positive ratio show 26.4 and 11.3% in fraction 3, and 66.4 and 88.2% in fraction 5, respectively.