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. 2021 Jun 11;11:691798. doi: 10.3389/fonc.2021.691798

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Copyright © 2021 Sun, Du, Kohli, Huang, Chen, Xu, Shen, Wang and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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DNA yield, size and overall view of genomic alterations in six fractions collected from a sequential centrifugation of 1 ml plasma sample. (A) DNA yield (ng) from six different fractions. (B) DNA size (bp) distribution in six plasma fractions of patient 1. (C) DNA size (bp) distribution in six plasma fractions of patient 7. (D) Overall view of genomic alterations in six plasma fractions and their corresponding whole plasma from patient 1. Segmentation-based copy number variation analysis shows different genomic variations across chromosomes 1–22. Most significant segments losses (arrows) on chromosome 6 were used to calculate ctDNA content. The log2 ratio scale in y axis was from −0.4 to 0.4. F1–6 represent fractions 1-6, respectively.