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. 2021 Jun 11;9:674260. doi: 10.3389/fbioe.2021.674260

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Copyright © 2021 Kahn-Krell, Pretorius, Ou, Fast, Litovsky, Berry, Liu and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Morphological characterization of CM spheroids. (A) Violin plot of spheroid size determined from bright field images on day 0, 12, and 30 of differentiation (n > 150). (B) Violin plot of cardiomyocyte yield on day 12 in monolayer and suspension culture systems determined as cells per volume of media (n = 4). (C) Bright field photos of differentiating spheroids over time. Scale bar = 500 μm. (D) H&E stained parafin sections of day 12 beating cardiomyocyte spheroids. Scale bar = 50 μm. (E) Flourescently stained cryosection showing cardiomyocytes muscle fiber striations. ^∗∗∗p < 0.001.