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. 2021 Jun 11;9:674260. doi: 10.3389/fbioe.2021.674260

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Copyright © 2021 Kahn-Krell, Pretorius, Ou, Fast, Litovsky, Berry, Liu and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Visual characterization of iCMs produced in suspension. (A) Day 12 cryosection stained for pMLKL showing no positive nuclei. (B) Day 12 cryosection with TUNEL staining and quantification of percent positive nuclei from examination of 5 spheroids. (C–G) Immunofluorescent antibody staining of day 12 suspension cardiomyocytes for cTnT and (C) SERCA, (D) Cx43, (E) JPH2, (F) MLC2v, and (G) MYL7 as (i) cryosections at low and high magnification and (ii) dissociated spheroids cultured for 3 days as monolayers.