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Analysis flow chart showing the screening process. The three cohorts including TCGA data set, GSE74777 data set (GEO), and independent cohort (National Cancer Center). LUSC, lung squamous cell carcinoma; TCGA, The Cancer Genome Atlas Program; GEO, gene expression omnibus database; LASSO least absolute shrinkage and selection operator; ESTIMATE, Estimation of STromal and Immune cells in MAlignant Tumor tissues using Expression data.
