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. 2021 Jun 11;12:673337. doi: 10.3389/fpls.2021.673337

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Copyright © 2021 Liu, Xia, Jiang, Shen and Pang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of the enzymatic efficiency of the recombinant LaPT2 protein toward different flavonoid substrates. (A) Chemical structures of the representative flavonoid substrates tested in the present study. (B) The conversion rate of the recombinant LaPT2 protein toward different flavonoid substrates. (C) Apparent Km values of the recombinant LaPT2 protein toward different flavonoid substrates. m/z is for mass-to-charge ratio, NI is for negative-ion mode, and PI is for positive-ion mode. The enzymatic assays were performed with technical triplicates in (B,C).