Figure 3.

Knockdown of SHARPIN expression decreased uterine sarcoma cell proliferation and colony formation. (A), Western blot analysis of SHARPIN expression in MES-SA cells transfected with negative control (NC) siNRA or SHARPIN siRNA for 72 h. (B), MES-SA cells were transfected with SHARPIN or NC siRNA for 72 h and plated in 96-well plates. Cell proliferation was analyzed daily by the CCK-8 assay during the following three days. Data were obtained from three independent experiments in triplicate. (C), Western blot analysis of SHARPIN expression in SK-UT-1 cells transfected with NC siNRA or SHARPIN siRNA for 72 h. (D), SK-UT-1 cells were transfected with SHARPIN or NC siRNA for 72 h and plated in 96-well plates. Cell proliferation was analyzed daily by the CCK-8 assay during the following three days. Data were obtained from three independent experiments performed in triplicate. *p < 0.05 vs. NC shRNA; n = 3. (E, F), Colony formation assay of MES-SA (E) and SK-UT-1 (F) cells after transfection with SHARPIN or NC siRNA for 72 h. *p < 0.05 vs. NC shRNA; n = 3. Full-length blots from Figures 3A, C are shown in Figure S5 .