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. 2021 Jun 11;12:673126. doi: 10.3389/fmicb.2021.673126

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Copyright © 2021 Carra, Russo, Micheli, Garbarino, Ricchi, Bergamini, Bassi, Prosperi, Piva, Cricca, Schiavo, Merialdi, Serraino and Arrigoni.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Neighbor-joining (NJ) tree. Genetic relationships among the 45 S. agalactiae sequence types (STs) obtained in the present study were inferred with MEGA5 software [14]. Evolutionary distances were calculated using the p-distance method, which considers the number of different nucleotides out of the total (3,456). A NJ tree with 1,000 bootstrap replications was constructed. The clonal complexes (CCs) obtained by the global eBURST analysis are reported.