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. 2021 Jun 11;8:689799. doi: 10.3389/fmolb.2021.689799

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Copyright © 2021 Apaolaza, Petropoulou and Rodriguez-Calvo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Two-dimensional spatial analysis of the localization and distance of immune cells to the islets. Heatmaps showing the distance of (A) CD3+ and (B) CD8+ cells to the closest islet. Scale: 0 (black-blue)—>250 (red) μm. (C) Bar graph showing the distribution of CD3+, (D) CD8+, and (E) CD4+ cells in five different distance categories to the closest islet: [0, 1), (1, 50], (50, 200], (200, 500], and >500 μm. (F) Violin plots showing the number of CD3+, (G) CD8+, and (H) CD4+ cells in the intra-islet [0, 1) and peri-islet (1, 50 μm]. Each dot represents a cell.