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. 2021 Jun 11;9:667879. doi: 10.3389/fcell.2021.667879

FIGURE 2.

FIGURE 2

RNA editing efficiencies of the CasRx system using different forms of single guide RNAs (sgRNA). (A) HEK293FT cells were transfected with the CasRx plasmid and short fragments of LacZ sgRNA (control), single vascular endothelial growth factor A (VEGFA) sgRNA, mixture of VEGFA sgRNAs (array), or VEGFA short hairpin (shRNA) for 72 h. (B) The VEGFA mRNA levels were determined by quantitative PCR (qPCR) as described in section “METHODS” (n = 6–12). (C) HEK293FT cells were transfected with the CasRx plasmid and non-targeting (control) pre-sgRNA, VEGFA pre-sgRNA, VEGFA array pre-sgRNA, and VEGFA shRNA for 72 h. (D) The VEGFA mRNA level was determined by qPCR as described in section “METHODS” (n = 5). Data are expressed as the mean ± SEM. Statistical analysis was performed with GraphPad Prism 7 (GraphPad, San Diego, CA, United States) and undertaken with one-way ANOVA and Tukey’s multiple comparison test. **P < 0.01, ***P < 0.001, ****P < 0.0001. Created with BioRender.com.